We reported that strontium (Sr) coating stimulated the osteogenic differentiation of bone marrow derived stromal cells (BMSC) in vitro. In this study we report an acceleration of bone formation by the apatite which contained silicon (Si) and Sr.
Material and method
We prepared polyethylene terephthalate (PET) films coated by four kinds of apatite. 1: hydroxyapatite (HAP) 2: Strontium carbonate apatite (SrCAP) 3: Calcium silicate apatite (CaSiP) 4: Strontium silicate apatite (SrSiP) We performed initial culture of BMSC derived from F 344 rats. Then we seeded 1× 104 cell/cm 2 MSCs on the films in 24 wells plate (n= 10). These were cultured for 14 days in osteoinductive medium. On the last day, we stocked conditioned medium to measure calcium concentration using an X-ray fluorescence spectrometer and secretory osteocalcin using an ELISA kit.
We performed statistical examination by one-way analysis of variance.
Calcium concentration in the medium significantly decreased in SrSiP and SrCAP as compared with HAP.
Osteocalcin concentration in the medium significantly increased in SrSiP as compared with HAP.
The osteogenesis was the most promoted on the apatite coated by SrSiP among the four kinds of apatite. We suggested that we can apply it to reforming of the materials surface of the artificial ligament in future.